- #1
Sunwoo Bae
- 60
- 4
- Homework Statement
- I am trying to analyze a western blot result of expression of Keratin Sulfate in variety of cells.
- Relevant Equations
- none
From this result, the conclusion is that MDCK-siNGT and MDCK-siB4Gal4 resulted in less keratin sulfate, while MDCK-RCA did not produce keratin sulfate at all. Looking at the gel, the band look very smeared, as if nothing was purified prior to blotting. I have noticed a similar looking gel in other articles as well, but is this a valid result?